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- 品牌：Invivogen
- 產(chǎn)地：國外
- 型號：詳詢
- 貨號：hctd-nfis
- 發(fā)布日期： 2023-03-31
- 更新日期： 2024-08-12
| 產(chǎn)地 | 國外 |
| 保存條件 | -20℃保存 |
| 品牌 | Invivogen |
| 貨號 | hctd-nfis |
| 用途 | 科研 |
| 組織來源 | 詳詢 |
| 細胞形態(tài) | 詳詢 |
| 是否是腫瘤細胞 | 是 |
| 保質期 | 詳詢 |
| 器官來源 | 詳詢 |
| 免疫類型 | 詳詢 |
| 品系 | 詳詢 |
| 生長狀態(tài) | 詳詢 |
| 物種來源 | 詳詢 |
| 包裝規(guī)格 | 詳詢 |
| 是否進口 | 是 |
HCT116-Dual™ cells are adherent epithelial cells that have been derived from the human HCT116 colorectal carcinoma cell line by stable integration of two inducible reporter constructs.
HCT116-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP--1 binding sites.
HCT116-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements.
As a result, HCT116-Dual™ cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase.
Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
